Source: National Railroad Passenger Corporation. "Lake Shore Limited." In Amtrak System Timetable, Updated January 2018, 63. , : National Railroad Passenger Corporation, 2018.
| Station | Mile | Location |
|---|---|---|
| New York - Penn Station | New York, New York | |
| Croton-Harmon | Croton-on-Hudson, New York | |
| Poughkeepsie | Poughkeepsie (city), New York | |
| Rhinecliff | Rhinebeck (town), New York | |
| Albany-Rensselaer | Rensselaer, New York | |
| Boston - South Station | Boston, Massachusetts | |
| Boston - Back Bay Station | Boston, Massachusetts | |
| Framingham | Framingham, Massachusetts | |
| Worcester | Worcester, Massachusetts | |
| Springfield | Springfield, Massachusetts | |
| Pittsfield | Pittsfield, Massachusetts | |
| Albany-Rensselaer | Rensselaer, New York | |
| Schenectady | Schenectady, New York | |
| Utica | New York | |
| Syracuse | New York | |
| Rochester | New York | |
| Buffalo-Depew | New York | |
| Erie | Pennsylvania | |
| Cleveland - Lakefront Station | Ohio | |
| Elyria (Lorain) | Ohio | |
| Sandusky | Ohio | |
| Toledo | Ohio | |
| Bryan | Ohio | |
| Waterloo (Fort Wayne) | Indiana | |
| Elkhart | Indiana | |
| South Bend | Indiana | |
| Chicago - Union Station | Illinois |